验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HeLa cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
27218-1-AP targets KIF2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | KIF2A fusion protein Ag25692 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | kinesin heavy chain member 2A |
Synonyms | HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2 |
Calculated Molecular Weight | 679 aa, 77 kDa |
Observed Molecular Weight | 80 kDa~100 kDa |
GenBank Accession Number | BC031828 |
Gene Symbol | KIF2A |
Gene ID (NCBI) | 3796 |
RRID | AB_2880805 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00139 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kinesin Family Member 2A (KIF2A), also known as Kinesin-2, is a member of the kinesin-13 superfamily protein. KIF2A is a microtubule-depolymerizing kinesin motor protein with essential roles in neural progenitor division and axonal pruning during brain development. KIF2A is involved in microtubule depolymerization at minus ends of the spindle to generate pole-migration forces for chromosome movement. KIF2A is a crucial factor in the invasion and metastasis of various tumors, such as gastric cancer(PMID: 32801897), hepatocellular carcinoma(PMID: 36277155).
实验方案
Product Specific Protocols | |
---|---|
WB protocol for KIF2A antibody 27218-1-AP | Download protocol |
IHC protocol for KIF2A antibody 27218-1-AP | Download protocol |
IF protocol for KIF2A antibody 27218-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |