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验证数据展示

  • IF Staining of HeLa using CL488-27218

    IF Staining of HeLa using CL488-27218

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KIF2A antibody (CL488-27218) at dilution of 1:200.

  • IF Staining of A549 using CL488-27218

    IF Staining of A549 using CL488-27218

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 KIF2A antibody (CL488-27218) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated KIF2A Polyclonal antibody

KIF2A Polyclonal Antibody for IF/ICC
Cat No. CL488-27218

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 27218-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27218 targets KIF2A in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 KIF2A fusion protein Ag25692 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin heavy chain member 2A
别名 HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2
计算分子量 679 aa, 77 kDa
观测分子量 80 kDa~100 kDa
GenBank蛋白编号BC031828
基因名称 KIF2A
Gene ID (NCBI) 3796
RRIDAB_3672809
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00139
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kinesin Family Member 2A (KIF2A), also known as Kinesin-2, is a member of the kinesin-13 superfamily protein. KIF2A is a microtubule-depolymerizing kinesin motor protein with essential roles in neural progenitor division and axonal pruning during brain development. KIF2A is involved in microtubule depolymerization at minus ends of the spindle to generate pole-migration forces for chromosome movement. KIF2A is a crucial factor in the invasion and metastasis of various tumors, such as gastric cancer(PMID: 32801897), hepatocellular carcinoma(PMID: 36277155).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KIF2A antibody CL488-27218Download protocol
Standard Protocols
Click here to view our Standard Protocols