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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KIF2A Polyclonal antibody

KIF2A Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27218

Print datasheet

Synonyms

HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2

验证数据展示

  • IF Staining of HeLa using CL488-27218

    IF Staining of HeLa using CL488-27218

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KIF2A antibody (CL488-27218) at dilution of 1:200.

  • IF Staining of A549 using CL488-27218

    IF Staining of A549 using CL488-27218

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 KIF2A antibody (CL488-27218) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inA549 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 27218-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27218 targets KIF2A in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen KIF2A fusion protein Ag25692 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin heavy chain member 2A
Synonyms HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2
Calculated Molecular Weight 679 aa, 77 kDa
Observed Molecular Weight 80 kDa~100 kDa
GenBank Accession NumberBC031828
Gene Symbol KIF2A
Gene ID (NCBI) 3796
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00139
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kinesin Family Member 2A (KIF2A), also known as Kinesin-2, is a member of the kinesin-13 superfamily protein. KIF2A is a microtubule-depolymerizing kinesin motor protein with essential roles in neural progenitor division and axonal pruning during brain development. KIF2A is involved in microtubule depolymerization at minus ends of the spindle to generate pole-migration forces for chromosome movement. KIF2A is a crucial factor in the invasion and metastasis of various tumors, such as gastric cancer(PMID: 32801897), hepatocellular carcinoma(PMID: 36277155).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KIF2A antibody CL488-27218Download protocol
Standard Protocols
Click here to view our Standard Protocols