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  • KD/KO Validated

KLF5 Polyclonal antibody

KLF5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 21017-1-AP

Print datasheet

Synonyms

GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2



经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Caco-2 cells, mouse colon tissue, human testis tissue, PC-3 cells, HT-1080 cells, mouse small intestine tissue, HCT 116 cells, COLO 320 cells, rat colon tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21017-1-AP targets KLF5 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen KLF5 fusion protein Ag15267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Kruppel-like factor 5 (intestinal)
Synonyms GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
RRIDAB_10696447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13887
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).

实验方案

Product Specific Protocols
WB protocol for KLF5 antibody 21017-1-APDownload protocol
IHC protocol for KLF5 antibody 21017-1-APDownload protocol
IF protocol for KLF5 antibody 21017-1-APDownload protocol
IP protocol for KLF5 antibody 21017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection

Authors - Jiakai Hou
humanWB

Cell Res

ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.

Authors - Shunrong Ji
humanWB,IHC

Oncogene

KLF5 regulates actin remodeling to enhance the metastasis of nasopharyngeal carcinoma

Authors - Zhenyu Yang
humanWB

Cell Death Dis

DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.

Authors - Rong-Jie Fu
  • KD Validated
humanWB,IHC

Aging (Albany NY)

Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.

Authors - Yilong Li
  • KD Validated
humanWB

Oncotarget

Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.

Authors - Xiaolong Wei
  • KD Validated