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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KLF5 Polyclonal antibody

KLF5 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-21017

Print datasheet

Synonyms

BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2

验证数据展示

  • IF Staining of HeLa using CL488-21017

    IF Staining of HeLa using CL488-21017

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KLF5 antibody (CL488-21017) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of A549 using CL488-21017

    FC experiment of A549 using CL488-21017

    1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Klf5 Polyclonal Antibody (CL488-21017)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 21017-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21017 targets KLF5 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen KLF5 fusion protein Ag15267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Kruppel-like factor 5 (intestinal)
Synonyms BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13887
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KLF5 antibody CL488-21017Download protocol
FC protocol for CL Plus 488 KLF5 antibody CL488-21017Download protocol
Standard Protocols
Click here to view our Standard Protocols