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验证数据展示

  • IF Staining of HeLa using CL488-21017

    IF Staining of HeLa using CL488-21017

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KLF5 antibody (CL488-21017) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of A549 using CL488-21017

    FC experiment of A549 using CL488-21017

    1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Klf5 Polyclonal Antibody (CL488-21017)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KLF5 Polyclonal antibody

KLF5 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-21017

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 21017-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21017 targets KLF5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen KLF5 fusion protein Ag15267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Kruppel-like factor 5 (intestinal)
Synonyms GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
RRIDAB_3672723
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13887
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KLF5 antibody CL488-21017Download protocol
FC protocol for CL Plus 488 KLF5 antibody CL488-21017Download protocol
Standard Protocols
Click here to view our Standard Protocols