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KLF5 Polyclonal antibody

KLF5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21017-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Caco-2 cells, mouse colon tissue, human testis tissue, PC-3 cells, HT-1080 cells, mouse small intestine tissue, HCT 116 cells, COLO 320 cells, rat colon tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21017-1-AP targets KLF5 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 KLF5 fusion protein Ag15267 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Kruppel-like factor 5 (intestinal)
别名 GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2
计算分子量 457 aa, 51 kDa
观测分子量 51-55 kDa
GenBank蛋白编号BC042131
基因名称 KLF5
Gene ID (NCBI) 688
RRIDAB_10696447
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13887
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).

实验方案

Product Specific Protocols
WB protocol for KLF5 antibody 21017-1-APDownload protocol
IHC protocol for KLF5 antibody 21017-1-APDownload protocol
IF protocol for KLF5 antibody 21017-1-APDownload protocol
IP protocol for KLF5 antibody 21017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection

Authors - Jiakai Hou
humanWB

Cell Res

ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.

Authors - Shunrong Ji
humanWB,IHC

Oncogene

KLF5 regulates actin remodeling to enhance the metastasis of nasopharyngeal carcinoma

Authors - Zhenyu Yang
humanWB

Cell Death Dis

DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.

Authors - Rong-Jie Fu
  • KD Validated
humanWB,IHC

Aging (Albany NY)

Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.

Authors - Yilong Li
  • KD Validated
humanWB

Oncotarget

Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.

Authors - Xiaolong Wei
  • KD Validated
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