验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, Caco-2 cells, mouse colon tissue, human testis tissue, PC-3 cells, HT-1080 cells, mouse small intestine tissue, HCT 116 cells, COLO 320 cells, rat colon tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21017-1-AP targets KLF5 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | KLF5 fusion protein Ag15267 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Kruppel-like factor 5 (intestinal) |
| Synonyms | GC-box-binding protein 2, Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2 |
| Calculated Molecular Weight | 457 aa, 51 kDa |
| Observed Molecular Weight | 51-55 kDa |
| GenBank Accession Number | BC042131 |
| Gene Symbol | KLF5 |
| Gene ID (NCBI) | 688 |
| RRID | AB_10696447 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13887 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF5 antibody 21017-1-AP | Download protocol |
| IHC protocol for KLF5 antibody 21017-1-AP | Download protocol |
| IF protocol for KLF5 antibody 21017-1-AP | Download protocol |
| IP protocol for KLF5 antibody 21017-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection | ||
Cell Res ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer. | ||
Oncogene KLF5 regulates actin remodeling to enhance the metastasis of nasopharyngeal carcinoma | ||
Cell Death Dis DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.
| ||
Aging (Albany NY) Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.
| ||
Oncotarget Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.
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