验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, COLO 320 cells, HCT 116 cells, HepG2 cells, HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, Ramos cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
60161-1-Ig targets MSH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | MSH2 fusion protein Ag7835 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) |
| Synonyms | MSH 2, 2H5F5 |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC021566 |
| Gene Symbol | MSH2 |
| Gene ID (NCBI) | 4436 |
| RRID | AB_10666855 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43246 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MSH2 antibody 60161-1-Ig | Download protocol |
| IHC protocol for MSH2 antibody 60161-1-Ig | Download protocol |
| IF protocol for MSH2 antibody 60161-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Immunol Res Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability. | ||
Sci Rep Cold atmospheric plasma stabilizes mismatch repair for effective, uniform treatment of diverse colorectal cancer cell types | ||
Transl Res Novel MSH2 and TSC2 variants in a Chinese family with Lynch syndrome and their synergistic impact in urothelial carcinoma |
