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  • Featured Product
  • KD/KO Validated

MSH2 Monoclonal antibody, PBS Only (Capture)

MSH2 Monoclonal Antibody for WB, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

2H5F5

Cat no : 60161-1-PBS

Print datasheet

Synonyms

MSH 2, 2H5F5

验证数据展示

  • WB analysis of HeLa using 60161-1-Ig (same clone as 60161-1-PBS)

    WB analysis of HeLa using 60161-1-Ig (same clone as 60161-1-PBS)

    WB results of MSH2 (60161-1-Ig, 1:1000) antibody with si-Control and si-MSH2 transfected HeLa cells.. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • Cytometric bead array standard curve of MP50235-1

    Cytometric bead array standard curve of MP50235-1

    Cytometric bead array standard curve of MP50235-1, MSH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60161-1-PBS. Detection antibody: 60161-2-PBS. Standard:Ag7835. Range: 0.781-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.

产品信息

60161-1-PBS targets MSH2 as part of a matched antibody pair:

MP50235-1: 60161-1-PBS capture and 60161-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity human
Immunogen MSH2 fusion protein Ag7835 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Synonyms MSH 2, 2H5F5
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC021566
Gene Symbol MSH2
Gene ID (NCBI) 4436
RRIDAB_10666855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43246
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.