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验证数据展示

  • WB analysis of HeLa using 60161-1-Ig (same clone as 60161-1-PBS)

    WB analysis of HeLa using 60161-1-Ig (same clone as 60161-1-PBS)

    WB results of MSH2 (60161-1-Ig, 1:1000) antibody with si-Control and si-MSH2 transfected HeLa cells.. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • IF Staining of A431 using 60161-1-Ig (same clone as 60161-1-PBS)

    IF Staining of A431 using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSH2 antibody (60161-1-Ig, Clone: 2H5F5 ) at dilution of 1:2277 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 60161-1-Ig (same clone as 60161-1-PBS)

    IF Staining of A431 using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSH2 antibody (60161-1-Ig, Clone: 2H5F5 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50235-1

    Sandwich ELISA standard curve of MP50235-1

    Sandwich ELISA standard curve of MP50235-1, Human MSH2 Monoclonal Matched Antibody Pair - PBS only. 60161-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7835. 60161-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL

  • Sample test of MP50235-1

    Sample test of MP50235-1

    The mean MSH2 concentration was determined to be 10.60 ng/mL in A375 cell extract based on a 2.0 mg/mL extract load and 17.80 ng/mL in A549 cell extract based on a 2.6 mg/mL extract load.

  • IHC staining of Ramos using 60161-1-Ig (same clone as 60161-1-PBS)

    IHC staining of Ramos using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 60161-1-Ig (MSH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • Cytometric bead array standard curve of MP50235-1

    Cytometric bead array standard curve of MP50235-1

    Cytometric bead array standard curve of MP50235-1, MSH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60161-1-PBS. Detection antibody: 60161-2-PBS. Standard:Ag7835. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO验证

MSH2 Monoclonal antibody, PBS Only (Capture)

MSH2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 60161-1-PBS

产品说明书

CloneNo. 2H5F5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

2H5F5, MSH 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.

产品信息

60161-1-PBS targets MSH2 as part of a matched antibody pair:

MP50235-1: 60161-1-PBS capture and 60161-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 MSH2 fusion protein Ag7835 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
别名 2H5F5, MSH 2
计算分子量 105 kDa
观测分子量 105 kDa
GenBank蛋白编号BC021566
基因名称 MSH2
Gene ID (NCBI) 4436
RRIDAB_10666855
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43246
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.