验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, NIH/3T3 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67996-1-Ig targets MYBBP1A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | MYBBP1A fusion protein Ag6008 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | MYB binding protein (P160) 1a |
Synonyms | FLJ37886, MYB binding protein (P160) 1a, Myb binding protein 1A, MYBBP1A, P160, PAP2 |
Calculated Molecular Weight | 149 kDa |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | BC050546 |
Gene Symbol | MYBBP1A |
Gene ID (NCBI) | 10514 |
RRID | AB_2918745 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9BQG0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].
实验方案
Product Specific Protocols | |
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WB protocol for MYBBP1A antibody 67996-1-Ig | Download protocol |
IHC protocol for MYBBP1A antibody 67996-1-Ig | Download protocol |
IF protocol for MYBBP1A antibody 67996-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |