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CD31 Polyclonal antibody
CD31 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (6)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA and More (3)
Conjugate
Unconjugated
372
验证数据展示
经过测试的应用
| Positive WB detected in | human peripheral blood platelets, HUVEC cells, Jurkat cells, THP-1 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue, human placenta tissue, human renal cell carcinoma tissue, human hepatocirrhosis tissue, human kidney tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human endometrial cancer tissue, human tonsillitis tissue |
| Positive IF/ICC detected in | HUVEC cells, human tonsillitis tissue, human endometrial cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11265-1-AP targets CD31 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP, Cell treatment |
| Tested Reactivity | human |
| Cited Reactivity | human, pig, rabbit, canine, monkey, zebrafish, goat |
| Immunogen | CD31 fusion protein Ag1787 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | platelet/endothelial cell adhesion molecule |
| Synonyms | PECAM1, PECAM 1, PECA1, GPIIA', GPIIA |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | BC022512 |
| Gene Symbol | CD31 |
| Gene ID (NCBI) | 5175 |
| ENSEMBL Gene ID | ENSG00000261371 |
| RRID | AB_2299349 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16284 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD31 antibody 11265-1-AP | Download protocol |
| IHC protocol for CD31 antibody 11265-1-AP | Download protocol |
| IF protocol for CD31 antibody 11265-1-AP | Download protocol |
| IP protocol for CD31 antibody 11265-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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