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CoraLite® Plus 488-conjugated CD31 Polyclonal antibody

CD31 Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL488-11265

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IF-P and More (1)

PECAM1, PECAM 1, PECA1, GPIIA', GPIIA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11265-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

CL488-11265 targets CD31 in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性human
免疫原 CD31 fusion protein Ag1787 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule
别名 PECAM1, PECAM 1, PECA1, GPIIA', GPIIA
计算分子量 83 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_2919023
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16284
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD31 antibody CL488-11265Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Biochem Biophys Res Commun

Dulaglutide restores endothelial progenitor cell levels in diabetic mice and mitigates high glucose-induced endothelial injury through SIRT1-mediated mitochondrial fission

Authors - Xi Mei
humanIF

FASEB J

CD74+ fibroblasts proliferate upon mechanical stretching to promote angiogenesis in keloids

Authors - Jingheng Zhang
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