验证数据展示
经过测试的应用
Positive IF-P detected in | human tonsillitis tissue |
Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 2 publications below |
产品信息
CL488-11265 targets CD31 in IF/ICC, IF-P applications and shows reactivity with human samples.
经测试应用 | IF/ICC, IF-P Application Description |
文献引用应用 | IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | CD31 fusion protein Ag1787 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | platelet/endothelial cell adhesion molecule |
别名 | PECAM1, PECAM 1, PECA1, GPIIA', GPIIA |
计算分子量 | 83 kDa |
GenBank蛋白编号 | BC022512 |
基因名称 | CD31 |
Gene ID (NCBI) | 5175 |
ENSEMBL Gene ID | ENSG00000261371 |
RRID | AB_2919023 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P16284 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CD31 antibody CL488-11265 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biochem Biophys Res Commun Dulaglutide restores endothelial progenitor cell levels in diabetic mice and mitigates high glucose-induced endothelial injury through SIRT1-mediated mitochondrial fission | ||
FASEB J CD74+ fibroblasts proliferate upon mechanical stretching to promote angiogenesis in keloids |