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PPAR Gamma Polyclonal antibody
PPAR Gamma Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF-P, FC (Intra), IP, ELISA and More (3)
Conjugate
Unconjugated
491
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HL-60 cells, mouse heart tissue, human heart tissue, MCF-7 cells, U-937 cells |
Positive IP detected in | HL-60 cells |
Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue, human breast cancer tissue, human placenta tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat liver tissue |
Positive FC (Intra) detected in | HeLa cells |
This antibody is not recommended for immunocytofluorescent assays.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16643-1-AP targets PPAR Gamma in WB, IHC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, CHIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, sheep, goat |
Immunogen | PPAR Gamma fusion protein Ag10005 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | peroxisome proliferator-activated receptor gamma |
Synonyms | PPARG, PPARγ, PPAR-gamma, Peroxisome proliferator-activated receptor gamma, NR1C3 |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 50-60 kDa |
GenBank Accession Number | BC006811 |
Gene Symbol | PPARG |
Gene ID (NCBI) | 5468 |
RRID | AB_10596794 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P37231 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PPAR Gamma antibody 16643-1-AP | Download protocol |
IHC protocol for PPAR Gamma antibody 16643-1-AP | Download protocol |
IF protocol for PPAR Gamma antibody 16643-1-AP | Download protocol |
IP protocol for PPAR Gamma antibody 16643-1-AP | Download protocol |
FC protocol for PPAR Gamma antibody 16643-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Nanotechnol Photoacoustic molecular imaging-escorted adipose photodynamic-browning synergy for fighting obesity with virus-like complexes. | ||
ACS Nano Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy. | ||
J Pineal Res Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin. | ||
Nat Commun N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism. | ||
Nat Commun METTL3 is essential for postnatal development of brown adipose tissue and energy expenditure in mice. | ||
Research (Wash D C) Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis |