验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
产品信息
15154-1-AP targets PSMB2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PSMB2 fusion protein Ag7198 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) subunit, beta type, 2 |
| 别名 | Proteasome subunit beta type-2, Proteasome component C7-I, Multicatalytic endopeptidase complex subunit C7-I, Macropain subunit C7-I |
| 计算分子量 | 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC000268 |
| 基因名称 | PSMB2 |
| Gene ID (NCBI) | 5690 |
| RRID | AB_2300322 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49721 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMB2, Proteasome subunit beta type-2, is also known as 20S proteasome subunit beta-4(PMID: 7918633). The proteasome is responsible for degradation of short lived and misfolded cytosolic and nuclear proteins in the cell and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMB2 is up-regulated in ovarian cancer cell lines. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD (vitamin A depletion) brain regions (PMID: 21190828). Knockdown of PSMB2 suppresses hepatocellular carcinoma and gastric cancer cell proliferation and invasion (PMID: 29780166, PMID: 36110152).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMB2 antibody 15154-1-AP | Download protocol |
| IF protocol for PSMB2 antibody 15154-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.
| ||
Mol Cancer Res Proteasome subunits differentially control Myeloma cell viability and proteasome inhibitor sensitivity. | ||
Front Pharmacol Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy. | ||
Metab Brain Dis Molecular alteration of the proteasome contributes to AD-like pathology in the brain of HFD-STZ diabetic rats | ||
Cell Mol Neurobiol Inhibition of the Proteasome Regulator PA28 Aggravates Oxidized Protein Overload in the Diabetic Rat Brain | ||
PLoS Biol Pharmacological induction of autophagy reduces inflammation in macrophages by degrading immunoproteasome subunits |
