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RAB10 Polyclonal antibody

RAB10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11808-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Ras-related protein Rab-10, Ras related protein Rab 10, EC:3.6.5.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, NIH/3T3 cells, rat brain tissue, C6 cells, HeLa cells, MCF-7 cells, MDCK cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy RAB10 Ready-To-Use IHC Kit. RAB10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11808-1-AP targets RAB10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, zebrafish
Immunogen RAB10 fusion protein Ag2473 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB10, member RAS oncogene family
Synonyms Ras-related protein Rab-10, Ras related protein Rab 10, EC:3.6.5.2
Calculated Molecular Weight 200 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC000896
Gene Symbol RAB10
Gene ID (NCBI) 10890
RRIDAB_2173442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61026
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB10 belongs to the RAS superfamily of small GTPases. RAB10 localizes to a system of tubules and vesicles, and it may be involved in vesicular trafficking and neurotransmitter release. It is also associated with Glut4 translocation in adipocytes.

实验方案

Product Specific Protocols
WB protocol for RAB10 antibody 11808-1-APDownload protocol
IHC protocol for RAB10 antibody 11808-1-APDownload protocol
IF protocol for RAB10 antibody 11808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IP,IF

Cell Res

MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development.

Authors - Xiao-Hui Xu
rat, zebrafishWB,IF

Nat Commun

Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development.

Authors - Liu Yang Y
mouseIF

Proc Natl Acad Sci U S A

Ras-related protein Rab10 facilitates TLR4 signaling by promoting replenishment of TLR4 onto the plasma membrane.

Authors - Wang Di D
mouseWB

Curr Biol

Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons

Authors - Sara C Sousa
ratWB

Sci Signal

Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.

Authors - Kong-Yan Wu
ratWB

J Neurosci

Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.

Authors - Pavlos Nathan J NJ
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