验证数据展示
经过测试的应用
Positive IF/ICC detected in | NIH/3T3 cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy RAB10 Ready-To-Use IHC Kit. RAB10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11808-1-AP
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-11808 targets RAB10 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat, Canine |
免疫原 | RAB10 fusion protein Ag2473 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RAB10, member RAS oncogene family |
别名 | RAB10, Ras related protein Rab 10 |
计算分子量 | 200 aa, 23 kDa |
观测分子量 | 23 kDa |
GenBank蛋白编号 | BC000896 |
基因名称 | RAB10 |
Gene ID (NCBI) | 10890 |
RRID | AB_3672514 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P61026 |
储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB10 belongs to the RAS superfamily of small GTPases. RAB10 localizes to a system of tubules and vesicles, and it may be involved in vesicular trafficking and neurotransmitter release. It is also associated with Glut4 translocation in adipocytes.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 RAB10 antibody CL488-11808 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |