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验证数据展示

  • WB analysis using 66058-1-Ig

    WB analysis using 66058-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of K562 using 66058-1-Ig

    WB analysis of K562 using 66058-1-Ig

    WB result of RAB27A antibody (66058-1-Ig, 1:5000) with si-Control and si-RAB27A transfected K562 cells.

  • IHC staining of human lymphoma using 66058-1-Ig

    IHC staining of human lymphoma using 66058-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human lymphoma using 66058-1-Ig

    IHC staining of human lymphoma using 66058-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB27A antibody included
  • Featured Product
  • KD/KO Validated

RAB27A Monoclonal antibody

RAB27A Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66058-1-Ig
CloneNo. 1C4B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K562, K-562 cells, Jurkat cells, HEK-293 cells, HL-60 cells, HepG2 cells
Positive IHC detected inhuman lymphoma tissue, human colon cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAB27A Ready-To-Use IHC Kit. RAB27A primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66058-1-Ig targets RAB27A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RAB27A fusion protein Ag12382 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB27A, member RAS oncogene family
Synonyms GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A
Calculated Molecular Weight 221 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC107680
Gene Symbol RAB27A
Gene ID (NCBI) 5873
RRIDAB_11042596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

实验方案

Product Specific Protocols
WB protocol for RAB27A antibody 66058-1-IgDownload protocol
IHC protocol for RAB27A antibody 66058-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols