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验证数据展示

  • WB analysis using 66058-1-Ig (same clone as 66058-1-PBS)

    WB analysis using 66058-1-Ig (same clone as 66058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • WB analysis of K562 using 66058-1-Ig (same clone as 66058-1-PBS)

    WB analysis of K562 using 66058-1-Ig (same clone as 66058-1-PBS)

    WB result of RAB27A antibody (66058-1-Ig, 1:5000) with si-Control and si-RAB27A transfected K562 cells. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB27A antibody included
  • Featured Product
  • KD/KO验证

RAB27A Monoclonal antibody, PBS Only

RAB27A Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66058-1-PBS

产品说明书

CloneNo. 1C4B8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RAB27A Ready-To-Use IHC Kit. RAB27A primary antibody included.

产品信息

66058-1-PBS targets RAB27A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 RAB27A fusion protein Ag12382 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB27A, member RAS oncogene family
别名 GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A
计算分子量 221 aa, 25 kDa
观测分子量 27 kDa
GenBank蛋白编号BC107680
基因名称 RAB27A
Gene ID (NCBI) 5873
RRIDAB_11042596
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP51159
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.