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  • Featured Product
  • KD/KO Validated

RAB27A Monoclonal antibody, PBS Only

RAB27A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C4B8

Cat no : 66058-1-PBS

Print datasheet

Synonyms

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

验证数据展示

  • WB analysis using 66058-1-Ig (same clone as 66058-1-PBS)

    WB analysis using 66058-1-Ig (same clone as 66058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • WB analysis of K562 using 66058-1-Ig (same clone as 66058-1-PBS)

    WB analysis of K562 using 66058-1-Ig (same clone as 66058-1-PBS)

    WB result of RAB27A antibody (66058-1-Ig, 1:5000) with si-Control and si-RAB27A transfected K562 cells. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB27A antibody included
  • IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB27A antibody included
Planning an IHC experiment? We recommend our IHCeasy RAB27A Ready-To-Use IHC Kit. RAB27A primary antibody included.

产品信息

66058-1-PBS targets RAB27A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen RAB27A fusion protein Ag12382 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB27A, member RAS oncogene family
Synonyms GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A
Calculated Molecular Weight 221 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC107680
Gene Symbol RAB27A
Gene ID (NCBI) 5873
RRIDAB_11042596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51159
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.