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  • KD/KO Validated

RAB8A Polyclonal antibody

RAB8A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 55296-1-AP

Print datasheet

Synonyms

MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A



经过测试的应用

Positive WB detected inA431 cells, mouse lung tissue, HepG2 cells, NIH/3T3 cells, human brain tissue, mouse skin tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55296-1-AP targets RAB8A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB8A, member RAS oncogene family
Synonyms MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberNM_005370
Gene Symbol RAB8A
Gene ID (NCBI) 4218
RRIDAB_10858398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61006
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.

实验方案

Product Specific Protocols
WB protocol for RAB8A antibody 55296-1-APDownload protocol
IHC protocol for RAB8A antibody 55296-1-APDownload protocol
IF protocol for RAB8A antibody 55296-1-APDownload protocol
IP protocol for RAB8A antibody 55296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineWB,IF

Nat Cell Biol

A molecular network for de novo generation of the apical surface and lumen.

Authors - Bryant David M DM
  • KD Validated
mouseWB,IF

Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu
mouseIF

Circ Res

Rasip1-Mediated Rho GTPase Signaling Regulates Blood Vessel Tubulogenesis via Nonmuscle Myosin II.

Authors - David M Barry
humanWB, IF

J Cell Biol

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

Authors - Michael C Burke
mouseIF

Nat Commun

Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis.

Authors - Shobi Veleri
humanWB

Proc Natl Acad Sci U S A

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Authors - Humbert Melissa C MC