MMP14 / MT1-MMP Polyclonal antibody
MMP14 / MT1-MMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, A549 cells, COLO 320 cells, human colon tissue |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, COLO 320 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14552-1-AP targets MMP14 / MT1-MMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | MMP14 / MT1-MMP fusion protein Ag5965 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | matrix metallopeptidase 14 (membrane-inserted) |
Synonyms | MMP14, Membrane-type-1 matrix metalloproteinase, Membrane-type matrix metalloproteinase 1, Matrix metalloproteinase-14, Matrix metalloproteinase 14 |
Calculated Molecular Weight | 66 kDa |
Observed Molecular Weight | 66 kDa, 45-50 kDa |
GenBank Accession Number | BC064803 |
Gene Symbol | MMP14 |
Gene ID (NCBI) | 4323 |
RRID | AB_2250751 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P50281 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MMP14 / MT1-MMP antibody 14552-1-AP | Download protocol |
IHC protocol for MMP14 / MT1-MMP antibody 14552-1-AP | Download protocol |
IF protocol for MMP14 / MT1-MMP antibody 14552-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Neuroinflammation SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury | ||
Mol Ther METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy. | ||
Int J Mol Sci PLOD2 Is a Prognostic Marker in Glioblastoma That Modulates the Immune Microenvironment and Tumor Progression. | ||
Int J Mol Sci LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases. | ||
Cancers (Basel) WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells. | ||
Cancer Lett Long non-coding RNA MALAT1 promotes gastric cancer tumorigenicity and metastasis by regulating vasculogenic mimicry and angiogenesis. |