验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RAF1 Ready-To-Use IHC Kit. RAF1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26863-1-AP targets RAF1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | RAF1 fusion protein Ag25423 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | v-raf-1 murine leukemia viral oncogene homolog 1 |
| Synonyms | Proto-oncogene c-RAF, Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF |
| Calculated Molecular Weight | 648 aa, 73 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC018119 |
| Gene Symbol | RAF1 |
| Gene ID (NCBI) | 5894 |
| RRID | AB_2880660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04049 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAF1 antibody 26863-1-AP | Download protocol |
| IHC protocol for RAF1 antibody 26863-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Intracellular magnetic hyperthermia reverses sorafenib resistance in hepatocellular carcinoma through its action on signaling pathways | ||
Virol Sin PLX8394, a RAF inhibitor, inhibits enterovirus 71 replication by blocking RAF/MEK/ERK signaling | ||
Arch Biochem Biophys Serum exosomal miRNA promote glioma progression by targeting SOS1 via abscopal effect of radiation | ||
Mol Cell Probes Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway | ||
FEBS Open Bio CXCR4 promotes gefitinib resistance of Huh7 cells by activating the c-Met signaling pathway. |
