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  • Featured Product
  • KD/KO Validated

RRM1 Polyclonal antibody

RRM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10526-1-AP

Print datasheet

Synonyms

RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit

验证数据展示

  • WB analysis of HeLa using 10526-1-AP

    WB analysis of HeLa using 10526-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10526-1-AP

    WB analysis using 10526-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 10526-1-AP

    IP experiment of K-562 using 10526-1-AP

    IP result of anti-RRM1 (IP:10526-1-AP, 5ug; Detection:10526-1-AP 1:800) with K-562 cells lysate 8000ug.

  • IHC staining of human breast cancer using 10526-1-AP

    IHC staining of human breast cancer using 10526-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RRM1 antibody included
  • IHC staining of human breast cancer using 10526-1-AP

    IHC staining of human breast cancer using 10526-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RRM1 antibody included
  • IF Staining of HepG2 using 10526-1-AP

    IF Staining of HepG2 using 10526-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM1 antibody (10526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • WB analysis of A549 using 10526-1-AP

    WB analysis of A549 using 10526-1-AP

    A549 cells (shcontrol and shRNA of RRM1) were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000. .

  • FC experiment of HeLa using 10526-1-AP

    FC experiment of HeLa using 10526-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RRM1 Polyclonal antibody (10526-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, A549 cells, K-562 cells, BxPC-3 cells, HEK-293 cells, U-251 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Raji cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10526-1-AP targets RRM1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, xenopus
Immunogen RRM1 fusion protein Ag0789 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonucleotide reductase M1
Synonyms RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC006498
Gene Symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_2180372
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23921
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleoside-diphosphate reductase large subunit (RRM1) is the main enzyme for de novo deoxyribonucleotide 

synthesis necessary for DNA synthesis during the cell cycle as well as during DNA repair.


What is the molecular weight of RRM1?

The molecular weight of RRM1 is 90 kDa. RRM1 is a part of ribonucleotide reductase (RNR), which is composed 

of two large RRM1 and two small RRM2 subunits in S phase and two large RRM1 and two alternative small p53R2 

subunits in non-dividing quiescent cells (PMID: 17416930).


What is the subcellular localization of RRM1?

RRM1 localizes to the cytoplasm, where it is responsible for dNTP production, forming a complex with RRM2. 

However, RRM1 can be recruited to DNA damage sites in the nucleus via interaction with Tip60 (PMID: 20159953).


What is the tissue expression pattern of RRM1?

RRM1 is ubiquitously expressed.


How is RRM1 expression regulated during the cell cycle and upon DNA damage?

Rrm1 gene expression levels depend on the cell cycle, and the highest mRNA levels are observed in S phase. 

However, RRM1 protein levels are constant throughout the cell cycle due to long protein half-life, contrary to 

RRM2, which is actively degraded after cell exit from S phase. During DNA damage, e.g., induced with genotoxins 

or UV light, expression of RRM1 and RRM2/p53R2 is upregulated (PMID: 1551913 and 8798592).


实验方案

Product Specific Protocols
WB protocol for RRM1 antibody 10526-1-APDownload protocol
IHC protocol for RRM1 antibody 10526-1-APDownload protocol
IF protocol for RRM1 antibody 10526-1-APDownload protocol
IP protocol for RRM1 antibody 10526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

N Engl J Med

DNA synthesis and repair genes RRM1 and ERCC1 in lung cancer.

Authors - Zhong Zheng
View Article
humanIF

Lancet Oncol

The ribonucleotide reductase large subunit (RRM1) as a predictive factor in patients with cancer.

Authors - Lars Petter Jordheim
View Article
humanWB

Clin Cancer Res

PMID: 25224279

Authors - Martin Kerr
  • KD Validated
View Article
humanWB, IHC, IP

Clin Cancer Res

PMID: 26228206

Authors - Francesca Vena
View Article
humanIHC

Ann Oncol

PMID: 23038758

Authors - Vilmar A C AC
View Article
IHC

Oncotarget

Impact of molecular profiling on overall survival of patients with advanced ovarian cancer.

Authors - Thomas J Herzog
View Article