验证数据展示
经过测试的应用
| Positive WB detected in | ROS1728 cells, MCF-7 cells |
| Positive IP detected in | ROS1728 cells |
| Positive IHC detected in | rat skin tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | ROS1728 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20700-1-AP targets RUNX2 in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, chIP |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | runt-related transcription factor 2 |
| Synonyms | OSF2, OSF 2, Core-binding factor subunit alpha-1, CBF-alpha-1, AML-3 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57-60 kDa |
| GenBank Accession Number | NM_004348 |
| Gene Symbol | RUNX2 |
| Gene ID (NCBI) | 860 |
| RRID | AB_2722783 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13950 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RUNX2 antibody 20700-1-AP | Download protocol |
| IHC protocol for RUNX2 antibody 20700-1-AP | Download protocol |
| IF protocol for RUNX2 antibody 20700-1-AP | Download protocol |
| IP protocol for RUNX2 antibody 20700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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