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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RUNX2 Polyclonal antibody

RUNX2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20700

Print datasheet

Synonyms

OSF2, OSF 2, Core-binding factor subunit alpha-1, CBF-alpha-1, AML-3

验证数据展示

  • IF Staining of SH-SY5Y using CL488-20700

    IF Staining of SH-SY5Y using CL488-20700

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 RUNX2 antibody (CL488-20700) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-20700

    FC experiment of HepG2 using CL488-20700

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RUNX2 (CL488-20700) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 20700-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20700 targets RUNX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name runt-related transcription factor 2
Synonyms OSF2, OSF 2, Core-binding factor subunit alpha-1, CBF-alpha-1, AML-3
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57-60 kDa
GenBank Accession NumberNM_004348
Gene Symbol RUNX2
Gene ID (NCBI) 860
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13950
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RUNX2 antibody CL488-20700Download protocol
FC protocol for CL Plus 488 RUNX2 antibody CL488-20700Download protocol
Standard Protocols
Click here to view our Standard Protocols