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CoraLite® Plus 488-conjugated RUNX2 Polyclonal antibody

RUNX2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-20700

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra) and More (1)

OSF2, OSF 2, Core-binding factor subunit alpha-1, CBF-alpha-1, AML-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 20700-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-20700 targets RUNX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 runt-related transcription factor 2
别名 OSF2, OSF 2, Core-binding factor subunit alpha-1, CBF-alpha-1, AML-3
计算分子量 57 kDa
观测分子量 57-60 kDa
GenBank蛋白编号NM_004348
基因名称 RUNX2
Gene ID (NCBI) 860
RRIDAB_3084039
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13950
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RUNX2 antibody CL488-20700Download protocol
FC protocol for CL Plus 488 RUNX2 antibody CL488-20700Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

J Lipid Res

sEV-mediated Lipid Droplets Transferred from Bone Marrow Adipocytes promote ferroptosis and impair Osteoblast Function

Authors - Weibo Huang
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