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- KD/KO Validated
RUNX2 Polyclonal antibody
RUNX2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (3)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
171
验证数据展示
经过测试的应用
Positive WB detected in | ROS1728 cells, MCF-7 cells |
Positive IP detected in | ROS1728 cells |
Positive IHC detected in | rat skin tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | ROS1728 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20700-1-AP targets RUNX2 in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, chIP |
Tested Reactivity | human, rat |
Cited Reactivity | human, mouse, rat, pig, chicken |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | runt-related transcription factor 2 |
Synonyms | OSF2, OSF 2, Core-binding factor subunit alpha-1, CBF-alpha-1, AML-3 |
Calculated Molecular Weight | 57 kDa |
Observed Molecular Weight | 57-60 kDa |
GenBank Accession Number | NM_004348 |
Gene Symbol | RUNX2 |
Gene ID (NCBI) | 860 |
RRID | AB_2722783 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13950 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RUNX2 antibody 20700-1-AP | Download protocol |
IHC protocol for RUNX2 antibody 20700-1-AP | Download protocol |
IF protocol for RUNX2 antibody 20700-1-AP | Download protocol |
IP protocol for RUNX2 antibody 20700-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
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