验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse heart tissue, HeLa cells, human skeletal muscle tissue, rat brain tissue, C6 cells, HEK-293 cells, K-562 cells, Jurkat cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human colon tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, hTERT-RPE1 cells and Mouse embryonic fibroblasts |
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11397-1-AP targets Septin 2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, canine |
| 免疫原 | Septin 2 fusion protein Ag1946 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | septin 2 |
| 别名 | NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5 |
| 计算分子量 | 41 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC014455 |
| 基因名称 | Septin 2 |
| Gene ID (NCBI) | 4735 |
| RRID | AB_2239110 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15019 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEPT2 belongs to a family of septins, GTP-binding proteins that are a component of the cytoskeleton. Septins can assemble into intracellular structures of various shapes and are known to influence membrane stability, forming diffusion barriers and serving as scaffolds.
What is the molecular weight of SEPT2? Is SEPT2 post-translationally modified?
The molecular weight of SEPT2 is 45 kDa. In a cellular context, SEPT2 forms hetero-hexamers and hetero-octamers with other septins. These hexamers and octamers further assemble into high-order structures such as filaments and rings. SEPT2 can be phosphorylated on Ser218 by casein kinase 2 (PMID: 19165576) and can also be SUMOylated (PMID: 29051266).
What is the subcellular localization of SEPT2?
SEPT2 forms a diffusion barrier and localizes to the primary cilium; in some cell types, it has been found at the transition zone/basal body (PMID: 20558667), while in others localized to the axoneme (PMID: 23572511). During cell division, SEPT2 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody (PMID: 9203580). In interphase cells, SEPT2 was found to associate with polyglutamylated microtubules (PMID: 18209106) and can affect microtubule dynamics via interaction with MAP4.
What is the tissue expression pattern of SEPT2?
While some septins have tissue-specific expression profiles, SEPT2 is ubiquitously expressed (PMID: 22314400).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 2 antibody 11397-1-AP | Download protocol |
| IHC protocol for Septin 2 antibody 11397-1-AP | Download protocol |
| IF protocol for Septin 2 antibody 11397-1-AP | Download protocol |
| IP protocol for Septin 2 antibody 11397-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells. | ||
PLoS Genet Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis. | ||
Cell Death Dis JMJD2C-mediated long non-coding RNA MALAT1/microRNA-503-5p/SEPT2 axis worsens non-small cell lung cancer. | ||
Elife Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction. | ||
PLoS Genet SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility. |
