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验证数据展示

  • FC experiment of MCF-7 using CL488-11397

    FC experiment of MCF-7 using CL488-11397

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Septin 2 (CL488-11397) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-11397

    IF Staining of HepG2 using CL488-11397

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Septin 2 antibody (CL488-11397) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Septin 2 Polyclonal antibody

Septin 2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11397

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11397-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11397 targets Septin 2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Septin 2 fusion protein Ag1946 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name septin 2
Synonyms NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC014455
Gene Symbol Septin 2
Gene ID (NCBI) 4735
RRIDAB_3083901
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15019
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEPT2 belongs to a family of septins, GTP-binding proteins that are a component of the cytoskeleton. Septins can assemble into intracellular structures of various shapes and are known to influence membrane stability, forming diffusion barriers and serving as scaffolds.

What is the molecular weight of SEPT2? Is SEPT2 post-translationally modified?

The molecular weight of SEPT2 is 45 kDa. In a cellular context, SEPT2 forms hetero-hexamers and hetero-octamers with other septins. These hexamers and octamers further assemble into high-order structures such as filaments and rings. SEPT2 can be phosphorylated on Ser218 by casein kinase 2 (PMID: 19165576) and can also be SUMOylated (PMID: 29051266).

What is the subcellular localization of SEPT2?

SEPT2 forms a diffusion barrier and localizes to the primary cilium; in some cell types, it has been found at the transition zone/basal body (PMID: 20558667), while in others localized to the axoneme (PMID: 23572511). During cell division, SEPT2 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody (PMID: 9203580). In interphase cells, SEPT2 was found to associate with polyglutamylated microtubules (PMID: 18209106) and can affect microtubule dynamics via interaction with MAP4.

What is the tissue expression pattern of SEPT2?

While some septins have tissue-specific expression profiles, SEPT2 is ubiquitously expressed (PMID: 22314400).


实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Septin 2 antibody CL488-11397Download protocol
Standard Protocols
Click here to view our Standard Protocols