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CoraLite® Plus 488-conjugated Septin 2 Polyclonal antibody

Septin 2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11397

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11397-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11397 targets Septin 2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Septin 2 fusion protein Ag1946 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 septin 2
别名 NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5
计算分子量 41 kDa
观测分子量 45 kDa
GenBank蛋白编号BC014455
基因名称 Septin 2
Gene ID (NCBI) 4735
RRIDAB_3083901
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15019
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEPT2 belongs to a family of septins, GTP-binding proteins that are a component of the cytoskeleton. Septins can assemble into intracellular structures of various shapes and are known to influence membrane stability, forming diffusion barriers and serving as scaffolds.

What is the molecular weight of SEPT2? Is SEPT2 post-translationally modified?

The molecular weight of SEPT2 is 45 kDa. In a cellular context, SEPT2 forms hetero-hexamers and hetero-octamers with other septins. These hexamers and octamers further assemble into high-order structures such as filaments and rings. SEPT2 can be phosphorylated on Ser218 by casein kinase 2 (PMID: 19165576) and can also be SUMOylated (PMID: 29051266).

What is the subcellular localization of SEPT2?

SEPT2 forms a diffusion barrier and localizes to the primary cilium; in some cell types, it has been found at the transition zone/basal body (PMID: 20558667), while in others localized to the axoneme (PMID: 23572511). During cell division, SEPT2 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody (PMID: 9203580). In interphase cells, SEPT2 was found to associate with polyglutamylated microtubules (PMID: 18209106) and can affect microtubule dynamics via interaction with MAP4.

What is the tissue expression pattern of SEPT2?

While some septins have tissue-specific expression profiles, SEPT2 is ubiquitously expressed (PMID: 22314400).


实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Septin 2 antibody CL488-11397Download protocol
Standard Protocols
Click here to view our Standard Protocols
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