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  • Featured Product
  • KD/KO Validated

Septin 2 Polyclonal antibody

Septin 2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11397-1-AP

Print datasheet

Synonyms

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

验证数据展示

  • WB analysis using 11397-1-AP

    WB analysis using 11397-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 11397-1-AP

    IHC staining of human liver cancer using 11397-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPTIN2 antibody included
  • IHC staining of human liver cancer using 11397-1-AP

    IHC staining of human liver cancer using 11397-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPTIN2 antibody included
  • IF Staining of HepG2 using 11397-1-AP

    IF Staining of HepG2 using 11397-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11397-1-AP (Septin 2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of hTERT-RPE1 cells and Mouse embryonic fibroblasts using 11397-1-AP

    IF Staining of hTERT-RPE1 cells and Mouse embryonic fibroblasts using 11397-1-AP

    IF result from Dr. Kevin Corbit. Septin 2 (11397-1-AP) marks the transition zone and cilium of Human hTERT-RPE1 cells and Mouse embryonic fibroblasts.

  • IP experiment of mouse brain using 11397-1-AP

    IP experiment of mouse brain using 11397-1-AP

    IP result of anti-Septin 2 (IP:11397-1-AP, 3ug; Detection:11397-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

  • WB analysis of HEK-293 using 11397-1-AP

    WB analysis of HEK-293 using 11397-1-AP

    WB result of Septin 2 antibody (11397-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 2 transfected HEK-293 cells.

  • IHC staining of human colon using 11397-1-AP

    IHC staining of human colon using 11397-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPTIN2 antibody included
  • IF Staining of HepG2 using 11397-1-AP

    IF Staining of HepG2 using 11397-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Septin 2 antibody (11397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of MCF-7 using 11397-1-AP

    FC experiment of MCF-7 using 11397-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Septin 2 (11397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, HeLa cells, human skeletal muscle tissue, rat brain tissue, C6 cells, HEK-293 cells, K-562 cells, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human colon tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, hTERT-RPE1 cells and Mouse embryonic fibroblasts
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11397-1-AP targets Septin 2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Septin 2 fusion protein Ag1946 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name septin 2
Synonyms NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC014455
Gene Symbol Septin 2
Gene ID (NCBI) 4735
RRIDAB_2239110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15019
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEPT2 belongs to a family of septins, GTP-binding proteins that are a component of the cytoskeleton. Septins can assemble into intracellular structures of various shapes and are known to influence membrane stability, forming diffusion barriers and serving as scaffolds.

What is the molecular weight of SEPT2? Is SEPT2 post-translationally modified?

The molecular weight of SEPT2 is 45 kDa. In a cellular context, SEPT2 forms hetero-hexamers and hetero-octamers with other septins. These hexamers and octamers further assemble into high-order structures such as filaments and rings. SEPT2 can be phosphorylated on Ser218 by casein kinase 2 (PMID: 19165576) and can also be SUMOylated (PMID: 29051266).

What is the subcellular localization of SEPT2?

SEPT2 forms a diffusion barrier and localizes to the primary cilium; in some cell types, it has been found at the transition zone/basal body (PMID: 20558667), while in others localized to the axoneme (PMID: 23572511). During cell division, SEPT2 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody (PMID: 9203580). In interphase cells, SEPT2 was found to associate with polyglutamylated microtubules (PMID: 18209106) and can affect microtubule dynamics via interaction with MAP4.

What is the tissue expression pattern of SEPT2?

While some septins have tissue-specific expression profiles, SEPT2 is ubiquitously expressed (PMID: 22314400).


实验方案

Product Specific Protocols
WB protocol for Septin 2 antibody 11397-1-APDownload protocol
IHC protocol for Septin 2 antibody 11397-1-APDownload protocol
IF protocol for Septin 2 antibody 11397-1-APDownload protocol
IP protocol for Septin 2 antibody 11397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells.

Authors - Pamela P Lee
View Article
humanWB,IF

J Cell Biol

The septin cytoskeleton regulates natural killer cell lytic granule release.

Authors - Prasad V Phatarpekar
View Article
mouseWB,IF

PLoS Genet

Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.

Authors - Manoj B Menon
View Article
human

Cell Death Dis

JMJD2C-mediated long non-coding RNA MALAT1/microRNA-503-5p/SEPT2 axis worsens non-small cell lung cancer.

Authors - Jun Zhang
View Article
mouseWB

Elife

Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.

Authors - Julia Patzig
View Article
mouseIF

PLoS Genet

SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.

Authors - Yi-Ru Shen
View Article