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验证数据展示

  • IF Staining of HepG2 using CL488-66410

    IF Staining of HepG2 using CL488-66410

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66410 (SIRT2 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SIRT2 Monoclonal antibody

SIRT2 Monoclonal Antibody for IF/ICC
Cat No. CL488-66410

Print datasheet

CloneNo. 1D8G10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥3200/ 100 μL

浓度: 1000 μg/ml

库存：现货

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SIRT2 Ready-To-Use IHC Kit. SIRT2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66410-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66410 targets SIRT2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen SIRT2 fusion protein Ag7756 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Synonyms SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 37-45 kDa
GenBank Accession NumberBC003547
Gene Symbol SIRT2
Gene ID (NCBI) 22933
RRIDAB_2883329
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IXJ6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. SIRT2 exists various isoforms and the molecular weight of isoforms are 35kDa, 40 kDa, and 42 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SIRT2 antibody CL488-66410Download protocol
Standard Protocols
Click here to view our Standard Protocols