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验证数据展示

  • IF Staining of HepG2 using CL488-66410

    IF Staining of HepG2 using CL488-66410

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66410 (SIRT2 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SIRT2 Monoclonal antibody

SIRT2 Monoclonal Antibody for IF/ICC
Cat No. CL488-66410

产品说明书

CloneNo. 1D8G10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC

别名

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SIRT2 Ready-To-Use IHC Kit. SIRT2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66410-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66410 targets SIRT2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig
免疫原 SIRT2 fusion protein Ag7756 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
别名 SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2
计算分子量 43 kDa
观测分子量 37-45 kDa
GenBank蛋白编号BC003547
基因名称 SIRT2
Gene ID (NCBI) 22933
RRIDAB_2883329
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8IXJ6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. SIRT2 exists various isoforms and the molecular weight of isoforms are 35kDa, 40 kDa, and 42 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SIRT2 antibody CL488-66410Download protocol
Standard Protocols
Click here to view our Standard Protocols