验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, L02 cells, LNCaP cells, K-562 cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67257-1-Ig targets SIRT5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | SIRT5 fusion protein Ag7743 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) |
| Synonyms | FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC000126 |
| Gene Symbol | SIRT5 |
| Gene ID (NCBI) | 23408 |
| RRID | AB_2882530 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NXA8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRT5 antibody 67257-1-Ig | Download protocol |
| IHC protocol for SIRT5 antibody 67257-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma | ||
EMBO J TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis. |
