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SSRP1 Monoclonal antibody

SSRP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67313-1-Ig
CloneNo. 3D3H4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit, Chromatin-specific transcription elongation factor 80 kDa subunit

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, 4T1 cells, NCCIT cells, PC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells, NIH/3T3 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SSRP1 Ready-To-Use IHC Kit. SSRP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67313-1-Ig targets SSRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SSRP1 fusion protein Ag8318 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name structure specific recognition protein 1
Synonyms FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit, Chromatin-specific transcription elongation factor 80 kDa subunit
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC005116
Gene Symbol SSRP1
Gene ID (NCBI) 6749
RRIDAB_2882573
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSRP1 antibody 67313-1-IgDownload protocol
IHC protocol for SSRP1 antibody 67313-1-IgDownload protocol
IF protocol for SSRP1 antibody 67313-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Biomed Pharmacother

CBL0137 activates ROS/BAX signaling to promote caspase-3/GSDME-dependent pyroptosis in ovarian cancer cells

Authors - Chang Yang
  • KD Validated
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