验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, 4T1 cells, NCCIT cells, PC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells, NIH/3T3 cells |
Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
67313-1-Ig targets SSRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | SSRP1 fusion protein Ag8318 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | structure specific recognition protein 1 |
Synonyms | FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit, Chromatin-specific transcription elongation factor 80 kDa subunit |
Calculated Molecular Weight | 81 kDa |
Observed Molecular Weight | 86 kDa |
GenBank Accession Number | BC005116 |
Gene Symbol | SSRP1 |
Gene ID (NCBI) | 6749 |
RRID | AB_2882573 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q08945 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for SSRP1 antibody 67313-1-Ig | Download protocol |
IHC protocol for SSRP1 antibody 67313-1-Ig | Download protocol |
IF protocol for SSRP1 antibody 67313-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |