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验证数据展示

  • IF Staining of HepG2 using CL488-67313

    IF Staining of HepG2 using CL488-67313

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SSRP1 antibody (CL488-67313, Clone: 3D3H4 ) at dilution of 1:100.

  • FC experiment of HeLa using CL488-67313

    FC experiment of HeLa using CL488-67313

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SSRP1 (CL488-67313, Clone:3D3H4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL488-67313

    IF Staining of HepG2 using CL488-67313

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SSRP1 antibody (CL488-67313, Clone: 3D3H4 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SSRP1 Monoclonal antibody

SSRP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67313

产品说明书

CloneNo. 3D3H4

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit, Chromatin-specific transcription elongation factor 80 kDa subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SSRP1 Ready-To-Use IHC Kit. SSRP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67313-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67313 targets SSRP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 SSRP1 fusion protein Ag8318 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 structure specific recognition protein 1
别名 FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit, Chromatin-specific transcription elongation factor 80 kDa subunit
计算分子量 81 kDa
GenBank蛋白编号BC005116
基因名称 SSRP1
Gene ID (NCBI) 6749
RRIDAB_2919448
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ08945
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SSRP1 antibody CL488-67313Download protocol
FC protocol for CL Plus 488 SSRP1 antibody CL488-67313Download protocol
Standard Protocols
Click here to view our Standard Protocols