验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, rat spleen tissue, NIH/3T3 cells, Jurkat cells, K-562 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67241-1-Ig targets Thrombospondin 1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | rat |
| Immunogen | Thrombospondin 1 fusion protein Ag29129 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | thrombospondin 1 |
| Synonyms | THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1 |
| Calculated Molecular Weight | 129 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | NM_003246 |
| Gene Symbol | Thrombospondin 1 |
| Gene ID (NCBI) | 7057 |
| RRID | AB_2882520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P07996 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Thrombospondin 1 antibody 67241-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Diabetes Metab Syndr Obes Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats | ||
Phytomedicine Protective effect of alizarin on vascular endothelial dysfunction via inhibiting the type 2 diabetes-induced synthesis of THBS1 and activating the AMPK signaling pathway |
