验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 67002-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67002 targets fetuin-B in IF/ICC applications and shows reactivity with human, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, pig |
| 免疫原 | fetuin-B fusion protein Ag12463 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | fetuin B |
| 别名 | FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11 |
| 计算分子量 | 382 aa, 42 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC074734 |
| 基因名称 | Fetuin B |
| Gene ID (NCBI) | 26998 |
| RRID | AB_2919403 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UGM5 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 fetuin-B antibody CL488-67002 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |