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CoraLite® Plus 488-conjugated fetuin-B Monoclonal antibody

fetuin-B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H7D11

Cat no : CL488-67002

Print datasheet

Synonyms

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

验证数据展示

  • IF Staining of HepG2 using CL488-67002

    IF Staining of HepG2 using CL488-67002

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 fetuin-B antibody (CL488-67002, Clone: 2H7D11 ) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67002-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67002 targets fetuin-B in IF/ICC applications and shows reactivity with human, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, pig
Immunogen fetuin-B fusion protein Ag12463 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name fetuin B
Synonyms FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11
Calculated Molecular Weight 382 aa, 42 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC074734
Gene Symbol Fetuin B
Gene ID (NCBI) 26998
RRIDAB_2919403
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UGM5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 fetuin-B antibody CL488-67002Download protocol
Standard Protocols
Click here to view our Standard Protocols