验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, SMMC-7721 cells, HuH-7 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11566-1-AP targets MED15 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | MED15 fusion protein Ag2143 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mediator complex subunit 15 |
| 别名 | Mediator of RNA polymerase II transcription subunit 15, CTG7A, CTG repeat protein 7a, CAG7A, ARC105 |
| 计算分子量 | 788 aa, 87 kDa |
| 观测分子量 | 105 kDa |
| GenBank蛋白编号 | BC017110 |
| 基因名称 | MED15 |
| Gene ID (NCBI) | 51586 |
| RRID | AB_2141975 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96RN5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED15 antibody 11566-1-AP | Download protocol |
| IHC protocol for MED15 antibody 11566-1-AP | Download protocol |
| IF protocol for MED15 antibody 11566-1-AP | Download protocol |
| IP protocol for MED15 antibody 11566-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals. | ||
Cell Rep Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF. | ||
Int J Cancer MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.
| ||
PLoS Genet Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans. | ||
J Biol Chem Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune-response genes. | ||
Am J Pathol Clinical and Molecular Implications of MED15 in Head and Neck Squamous Cell Carcinoma. |
