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验证数据展示

  • IF Staining of SH-SY5Y using CL488-11566

    IF Staining of SH-SY5Y using CL488-11566

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 MED15 antibody (CL488-11566) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MED15 Polyclonal antibody

MED15 Polyclonal Antibody for IF/ICC
Cat No. CL488-11566

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

ARC105, CAG7A, CTG repeat protein 7a, CTG7A, DKFZp686A2214, DKFZp762B1216, FLJ42282, FLJ42935, MED15, mediator complex subunit 15, PCQAP, TIG 1, TIG1, TNRC7, TPA inducible gene 1 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 11566-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11566 targets MED15 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 MED15 fusion protein Ag2143 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator complex subunit 15
别名 ARC105, CAG7A, CTG repeat protein 7a, CTG7A, DKFZp686A2214, DKFZp762B1216, FLJ42282, FLJ42935, MED15, mediator complex subunit 15, PCQAP, TIG 1, TIG1, TNRC7, TPA inducible gene 1 protein
计算分子量 788 aa, 87 kDa
观测分子量 105 kDa
GenBank蛋白编号BC017110
基因名称 MED15
Gene ID (NCBI) 51586
RRIDAB_3083905
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RN5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MED15 antibody CL488-11566Download protocol
Standard Protocols
Click here to view our Standard Protocols