验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse testis tissue, HEK-293 cells, human brain tissue, rat testis tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells, hTERT-RPE1 cells, MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11744-1-AP targets IFT81 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | IFT81 fusion protein Ag2339 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | intraflagellar transport 81 homolog (Chlamydomonas) |
| 别名 | CDV1R, CDV-1, CDV1, CDV 1R, CDV 1 |
| 计算分子量 | 676 aa, 80 kDa |
| 观测分子量 | 75-80 kDa |
| GenBank蛋白编号 | BC029349 |
| 基因名称 | IFT81 |
| Gene ID (NCBI) | 28981 |
| RRID | AB_2121966 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WYA0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFT81 antibody 11744-1-AP | Download protocol |
| IHC protocol for IFT81 antibody 11744-1-AP | Download protocol |
| IF protocol for IFT81 antibody 11744-1-AP | Download protocol |
| IP protocol for IFT81 antibody 11744-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. | ||
Nat Cell Biol The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment. | ||
Nat Cell Biol Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. | ||
Nat Commun IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. | ||
Nat Commun Cilia locally synthesize proteins to sustain their ultrastructure and functions. | ||
EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. |
