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验证数据展示

  • IF Staining of MDCK using CL488-11744

    IF Staining of MDCK using CL488-11744

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • IF Staining of MDCK using CL488-11744

    IF Staining of MDCK using CL488-11744

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:50, CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

  • IF Staining of hTERT-RPE1 using CL488-11744

    IF Staining of hTERT-RPE1 using CL488-11744

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT81 antibody (CL488-11744) at dilution of 1:200, acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated IFT81 Polyclonal antibody

IFT81 Polyclonal Antibody for IF/ICC
Cat No. CL488-11744

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

IF/ICC

别名

CDV 1, CDV 1R, CDV1, CDV1R, IFT81

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells
For other applications, we recommend the unconjugated version of this antibody, 11744-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11744 targets IFT81 in IF/ICC applications and shows reactivity with human, canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, canine
免疫原 IFT81 fusion protein Ag2339 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intraflagellar transport 81 homolog (Chlamydomonas)
别名 CDV 1, CDV 1R, CDV1, CDV1R, IFT81
计算分子量 676 aa, 80 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC029349
基因名称 IFT81
Gene ID (NCBI) 28981
RRIDAB_2919039
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WYA0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IFT81 antibody CL488-11744Download protocol
Standard Protocols
Click here to view our Standard Protocols