验证数据展示
经过测试的应用
| Positive IF/ICC detected in | hTERT-RPE1 cells, MDCK cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-11744 targets IFT81 in IF/ICC applications and shows reactivity with human, canine samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, canine |
| Immunogen | IFT81 fusion protein Ag2339 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | intraflagellar transport 81 homolog (Chlamydomonas) |
| Synonyms | CDV 1, CDV 1R, CDV1, CDV1R, IFT81 |
| Calculated Molecular Weight | 676 aa, 80 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC029349 |
| Gene Symbol | IFT81 |
| Gene ID (NCBI) | 28981 |
| RRID | AB_2919039 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WYA0 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 IFT81 antibody CL488-11744 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |