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验证数据展示

  • WB analysis of human brain using 12150-1-AP

    WB analysis of human brain using 12150-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12150-1-AP (PTS antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12150-1-AP

    IHC staining of human gliomas using 12150-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12150-1-AP (PTS antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HeLa using 12150-1-AP

    IF Staining of HeLa using 12150-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTS antibody (12150-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

PTS Polyclonal antibody

PTS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12150-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

PTPS, PTP synthase, EC:4.2.3.12, 6-pyruvoyl tetrahydrobiopterin synthase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12150-1-AP targets PTS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 PTS fusion protein Ag2798 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 6-pyruvoyltetrahydropterin synthase
别名 PTPS, PTP synthase, EC:4.2.3.12, 6-pyruvoyl tetrahydrobiopterin synthase
计算分子量 145 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC009686
基因名称 PTS
Gene ID (NCBI) 5805
RRIDAB_2173398
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03393
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTS antibody 12150-1-APDownload protocol
IHC protocol for PTS antibody 12150-1-APDownload protocol
IF protocol for PTS antibody 12150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia.

Authors - Qin Zhao
View Article
mouseWB

Acta Physiol (Oxf)

Tetrahydrobiopterin synthesis and metabolism is impaired in dystrophin-deficient mdx mice and humans.

Authors - Angus Lindsay
View Article
humanWB,IHC

Transl Lung Cancer Res

PTS is activated by ATF4 and promotes lung adenocarcinoma development via the Wnt pathway

Authors - Wei Ma
  • KD Validated
View Article