验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17894-1-AP targets VPS15 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | VPS15 fusion protein Ag12221 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase, regulatory subunit 4 |
| 别名 | PI3K p150, PIK3R4, PI3-kinase p150 subunit, Phosphoinositide 3-kinase regulatory subunit 4, Phosphoinositide 3-kinase adaptor protein |
| 计算分子量 | 1358 aa, 153 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC110318 |
| 基因名称 | PIK3R4 |
| Gene ID (NCBI) | 30849 |
| RRID | AB_2163630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99570 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS15 antibody 17894-1-AP | Download protocol |
| IF protocol for VPS15 antibody 17894-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Neurosci Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. | ||
Nat Commun IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation. | ||
Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. | ||
J Hazard Mater Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria. | ||
Cancer Lett Targeting AR-Beclin 1 Complex-Modulated Growth Factor Signaling Increases the Antiandrogen-Enzalutamide Sensitivity to Better Suppress the Castration-Resistant Prostate Cancer Growth. |
