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CoraLite® Plus 488-conjugated VPS15 Polyclonal antibody

VPS15 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-17894

Print datasheet

Synonyms

p150, PI3 kinase p150 subunit, PI3K p150, PI3-kinase regulatory subunit 4, PIK3R4, VPS15

验证数据展示

  • IF Staining of HeLa using CL488-17894

    IF Staining of HeLa using CL488-17894

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 VPS15 antibody (CL488-17894) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-17894

    FC experiment of HepG2 using CL488-17894

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated VPS15 Polyclonal antibody (CL488-17894)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 17894-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17894 targets VPS15 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen VPS15 fusion protein Ag12221 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoinositide-3-kinase, regulatory subunit 4
Synonyms p150, PI3 kinase p150 subunit, PI3K p150, PI3-kinase regulatory subunit 4, PIK3R4, VPS15
Calculated Molecular Weight 1358 aa, 153 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC110318
Gene Symbol PIK3R4
Gene ID (NCBI) 30849
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99570
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VPS15 antibody CL488-17894Download protocol
Standard Protocols
Click here to view our Standard Protocols