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验证数据展示

  • WB analysis using 25780-1-AP

    WB analysis using 25780-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25780-1-AP (MTCL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 25780-1-AP

    IP experiment of A549 using 25780-1-AP

    IP result of anti-KIAA0802 (IP:25780-1-AP, 4ug; Detection:25780-1-AP 1:400) with A549 cells lysate 640 ug.

MTCL1 Polyclonal antibody

MTCL1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25780-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

WB, IP, ELISA

别名

SOGA2, KIAA0802, CCDC165

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MDCK cells
Positive IP detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25780-1-AP targets MTCL1 in WB, IP, ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, canine
免疫原 MTCL1 fusion protein Ag22718 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0802
别名 SOGA2, KIAA0802, CCDC165
计算分子量 1905 aa, 209 kDa
观测分子量250 kDa
GenBank蛋白编号BC040542
基因名称 MTCL1
Gene ID (NCBI) 23255
RRIDAB_3669499
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4B5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Microtubule cross‐linking factor 1 (MTCL1) is a MT‐regulating protein that plays essential roles in accumulating the non-centrosomal MTs into the apicobasal MT bundles. MTCL1, identified as a binding protein of the cell polarity‐regulating kinase, PAR‐1b/MARK2, is recruited to the Golgi membranes through interactions with CLASPs and AKAP450/CG-NAP, and promotes microtubule growth from the Golgi membrane. Western blot analysis detected MTCL1 at an apparent molecular mass of 250 kDa as indicated in the literature (PMID: 23902687, 28283581).

实验方案

Product Specific Protocols
WB protocol for MTCL1 antibody 25780-1-APDownload protocol
IP protocol for MTCL1 antibody 25780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols