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验证数据展示

  • WB analysis of HEK-293T using 27151-1-AP

    WB analysis of HEK-293T using 27151-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27151-1-AP (DOCK4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 27151-1-AP

    IF Staining of HeLa using 27151-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27151-1-AP (DOCK4 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

DOCK4 Polyclonal antibody

DOCK4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27151-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

dedicator of cytokinesis 4, DOCK4, KIAA0716

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DOCK4 Ready-To-Use IHC Kit. DOCK4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27151-1-AP targets DOCK4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 DOCK4 fusion protein Ag25946 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dedicator of cytokinesis 4
别名 dedicator of cytokinesis 4, DOCK4, KIAA0716
计算分子量 2011 aa, 230 kDa
观测分子量 225 kDa
GenBank蛋白编号BC117689
基因名称 DOCK4
Gene ID (NCBI) 9732
RRIDAB_2880776
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N1I0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DOCK4 antibody 27151-1-APDownload protocol
IF protocol for DOCK4 antibody 27151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols