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验证数据展示

  • IF Staining of HeLa using CL488-27151

    IF Staining of HeLa using CL488-27151

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DOCK4 antibody (CL488-27151) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-27151

    FC experiment of HeLa using CL488-27151

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DOCK4 (CL488-27151)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated DOCK4 Polyclonal antibody

DOCK4 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-27151

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

KIAA0716, dedicator of cytokinesis 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DOCK4 Ready-To-Use IHC Kit. DOCK4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27151-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27151 targets DOCK4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 DOCK4 fusion protein Ag25946 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dedicator of cytokinesis 4
别名 KIAA0716, dedicator of cytokinesis 4
计算分子量 2011 aa, 230 kDa
观测分子量 225 kDa
GenBank蛋白编号BC117689
基因名称 DOCK4
Gene ID (NCBI) 9732
RRIDAB_3672807
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N1I0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DOCK4 antibody CL488-27151Download protocol
FC protocol for CL Plus 488 DOCK4 antibody CL488-27151Download protocol
Standard Protocols
Click here to view our Standard Protocols