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GFRAL Polyclonal antibody

GFRAL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28335-1-AP

Print datasheet

Synonyms

UNQ9356, GRAL, C6orf144, bA360D14.1

验证数据展示

  • WB analysis using 28335-1-AP

    WB analysis using 28335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28335-1-AP (GFRAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse cerebellum tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28335-1-AP targets GFRAL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GFRAL fusion protein Ag28504 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GDNF family receptor alpha like
Synonyms UNQ9356, GRAL, C6orf144, bA360D14.1
Calculated Molecular Weight 45 aa, 85 kDa
Observed Molecular Weight 45-60 kDa
GenBank Accession NumberNM_207410
Gene Symbol GFRAL
Gene ID (NCBI) 389400
RRIDAB_3669653
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFRAL (GDNF family receptor alpha-like), also called C6orf144, is the brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses (PMID: 28846097, 28846098, 28846099, 28953886, 36630958). The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues (PMID: 32661391). GFRAL is a single-pass membrane protein, and localizes to the plasma membrane. It is mainly expressed in the hindbrain, and its mainstream molecular weight is 45-60 kd.

实验方案

Product Specific Protocols
WB protocol for GFRAL antibody 28335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols