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PRKAR2B Polyclonal antibody

PRKAR2B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 28351-1-AP

Print datasheet

Synonyms

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

验证数据展示

  • WB analysis using 28351-1-AP

    WB analysis using 28351-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of Neuro-2a using 28351-1-AP

    IF Staining of Neuro-2a using 28351-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of Neuro-2a using 28351-1-AP

    IF Staining of Neuro-2a using 28351-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of mouse brain using 28351-1-AP

    IP experiment of mouse brain using 28351-1-AP

    IP result of anti-PRKAR2B (IP:28351-1-AP, 4ug; Detection:28351-1-AP 1:1000) with mouse brain tissue lysate 1080 ug.

  • IF Staining of SH-SY5Y using 28351-1-AP

    IF Staining of SH-SY5Y using 28351-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of SH-SY5Y using 28351-1-AP

    FC experiment of SH-SY5Y using 28351-1-AP

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PRKAR2B (28351-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human stomach using 28351-1-AP

    IHC staining of human stomach using 28351-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 28351-1-AP

    IHC staining of human stomach cancer using 28351-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inY79 cells, human testis tissue, mouse brain tissue, mouse eye tissue, rat brain tissue, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells, SH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28351-1-AP targets PRKAR2B in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PRKAR2B fusion protein Ag28787 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, cAMP-dependent, regulatory, type II, beta
Synonyms RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit
Calculated Molecular Weight 418 aa, 46 kDa
Observed Molecular Weight46-50 kDa
GenBank Accession NumberBC075800
Gene Symbol PRKAR2B
Gene ID (NCBI) 5577
RRIDAB_2918156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31323
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRKAR2B antibody 28351-1-APDownload protocol
IHC protocol for PRKAR2B antibody 28351-1-APDownload protocol
IF protocol for PRKAR2B antibody 28351-1-APDownload protocol
IP protocol for PRKAR2B antibody 28351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Inflamm Res

Comprehensive Analysis of Fatty Acid Metabolism in Diabetic Nephropathy from the Perspective of Immune Landscapes, Diagnosis and Precise Therapy

Authors - Enyi Zhu
View Article