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验证数据展示

  • WB analysis using 60131-1-Ig (same clone as 60131-1-PBS)

    WB analysis using 60131-1-Ig (same clone as 60131-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

    IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NENF antibody included
  • IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

    IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NENF antibody included
  • IHC staining of human colon cancer using 60131-1-Ig (same clone as 60131-1-PBS)

    IHC staining of human colon cancer using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NENF antibody included
  • IF Staining of HeLa using 60131-1-Ig (same clone as 60131-1-PBS)

    IF Staining of HeLa using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Neudesin/NENF antibody (60131-1-Ig, Clone: 4G9E12 ) at dilution of 1:1900 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60131-1-Ig (same clone as 60131-1-PBS)

    IF Staining of HepG2 using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 60131-1-Ig(NENF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50685-1

    Cytometric bead array standard curve of MP50685-1

    Cytometric bead array standard curve of MP50685-1, NENF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60131-1-PBS. Detection antibody: 60131-2-PBS. Standard:Ag8387. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50685-2

    Cytometric bead array standard curve of MP50685-2

    Cytometric bead array standard curve of MP50685-2, NENF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60131-1-PBS. Detection antibody: 60131-3-PBS. Standard:Ag8387. Range: 0.098-100 ng/mL.

Neudesin/NENF Monoclonal antibody, PBS Only (Capture)

Neudesin/NENF Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 60131-1-PBS

产品说明书

CloneNo. 4G9E12

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor, Cell immortalization-related protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NENF Ready-To-Use IHC Kit. NENF primary antibody included.

产品信息

60131-1-PBS targets Neudesin/NENF as part of a matched antibody pair:

MP50685-1: 60131-1-PBS capture and 60131-2-PBS detection (validated in Cytometric bead array)

MP50685-2: 60131-1-PBS capture and 60131-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 Neudesin/NENF fusion protein Ag8387 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 neuron derived neurotrophic factor
别名 Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor, Cell immortalization-related protein 2
计算分子量 172 aa, 19 kDa
观测分子量 19 kDa, 16 kDa
GenBank蛋白编号BC008823
基因名称 NENF
Gene ID (NCBI) 29937
RRIDAB_2150987
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UMX5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).