验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, K-562 cells, pig liver tissue, rat liver tissue, HepG2 cells, HuH-7 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60171-1-Ig targets ALDH1A1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 | ALDH1A1 fusion protein Ag8551 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | aldehyde dehydrogenase 1 family, member A1 |
| 别名 | ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC, 3-deoxyglucosone dehydrogenase |
| 计算分子量 | 501 aa, 55 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC001505 |
| 基因名称 | ALDH1A1 |
| Gene ID (NCBI) | 216 |
| RRID | AB_10693634 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P00352 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALDH1A1 antibody 60171-1-Ig | Download protocol |
| IHC protocol for ALDH1A1 antibody 60171-1-Ig | Download protocol |
| IF protocol for ALDH1A1 antibody 60171-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. | ||
Cell Rep Med Targeting EMSY-mediated methionine metabolism is a potential therapeutic strategy for triple-negative breast cancer | ||
Elife Perirenal adipose tissue contains a subpopulation of cold-inducible adipocytes derived from brown-to-white conversion | ||
iScience Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression
| ||
Front Pharmacol Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells. | ||
Oral Dis microRNA-17 is a tumor suppressor in oral squamous cell carcinoma and is repressed by LSD1. |
