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验证数据展示

  • IF Staining of HeLa using 60171-1-Ig (same clone as 60171-1-PBS)

    IF Staining of HeLa using 60171-1-Ig (same clone as 60171-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDH1A1 antibody (60171-1-Ig, Clone: 1A10A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

  • WB analysis using 60171-1-Ig (same clone as 60171-1-PBS)

    WB analysis using 60171-1-Ig (same clone as 60171-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 60171-1-Ig (same clone as 60171-1-PBS)

    IHC staining of human brain using 60171-1-Ig (same clone as 60171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1A1 antibody included
  • IHC staining of human brain using 60171-1-Ig (same clone as 60171-1-PBS)

    IHC staining of human brain using 60171-1-Ig (same clone as 60171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1A1 antibody included
  • FC experiment of HepG2 using 60171-1-Ig (same clone as 60171-1-PBS)

    FC experiment of HepG2 using 60171-1-Ig (same clone as 60171-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1 (60171-1-Ig, Clone:1A10A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 60171-1-Ig (same clone as 60171-1-PBS)

    WB analysis of K-562 using 60171-1-Ig (same clone as 60171-1-PBS)

    WB result of ALDH1A1 antibody (60171-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1A1 transfected K-562 cells. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ALDH1A1 Monoclonal antibody, PBS Only

ALDH1A1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 60171-1-PBS

产品说明书

CloneNo. 1A10A2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC, 3-deoxyglucosone dehydrogenase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ALDH1A1 Ready-To-Use IHC Kit. ALDH1A1 primary antibody included.

产品信息

60171-1-PBS targets ALDH1A1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ALDH1A1 fusion protein Ag8551 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aldehyde dehydrogenase 1 family, member A1
别名 ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC, 3-deoxyglucosone dehydrogenase
计算分子量 501 aa, 55 kDa
观测分子量 52 kDa
GenBank蛋白编号BC001505
基因名称 ALDH1A1
Gene ID (NCBI) 216
RRIDAB_10693634
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00352
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).