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  • Featured Product
  • KD/KO Validated

CDX2 Monoclonal antibody, PBS Only (Capture)

CDX2 Monoclonal Antibody for WB, IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

5A4E6

Cat No : 60243-1-PBS

Print datasheet

Synonyms

caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX-2, CDX3, Homeobox protein CDX 2

验证数据展示

  • WB analysis of COLO 320 using 60243-1-Ig (same clone as 60243-1-PBS)

    WB analysis of COLO 320 using 60243-1-Ig (same clone as 60243-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

  • IF Staining of Caco-2 using 60243-1-Ig (same clone as 60243-1-PBS)

    IF Staining of Caco-2 using 60243-1-Ig (same clone as 60243-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (60243-1-Ig, Clone: 5A4E6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50121-1

    Cytometric bead array standard curve of MP50121-1

    Cytometric bead array standard curve of MP50121-1, CDX2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60243-1-PBS. Detection antibody: 60243-3-PBS. Standard:ag17644. Range: 0.098-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CDX2 Ready-To-Use IHC Kit. CDX2 primary antibody included.

产品信息

60243-1-PBS targets CDX2 as part of a matched antibody pair:

MP50121-1: 60243-1-PBS capture and 60243-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity human, pig
Immunogen CDX2 fusion protein Ag17644 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caudal type homeobox 2
Synonyms caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX-2, CDX3, Homeobox protein CDX 2
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberBC014461
Gene Symbol CDX2
Gene ID (NCBI) 1045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99626
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.